$890.00 CAD
IFlowPlate is an organ on a chip platform designed to replicate the complex microenvironment of human tissues with interstitial flow. Each IFlowPlate consists of 128 independent tissue culture units, offering the highest throughput available in the market and compatibility with automation workflows. Each unit includes an inlet, middle, and outlet well, interconnected by two channels. The simplistic design makes the platform highly versatile. To create an epithelial tissue barrier model, a hydrogel can be cast in the middle well, with epithelial cells seeded on the gel surface and supporting cells embedded within. For controlling epithelial topography, TopoStamp (B003) introduces specific patterns on the gel surface. For vascularized organoids, endothelial cells and organoids can be embedded in the gel.
For simple perfusion cultures of free floating organoids or tissue spheroids, the organoids can be directly seeded into the middle wells without a gel. The dimensions of the inlet and outlet channels are adjustable for specific applications. Narrower channels (A001) are ideal for physically connecting to self assembled vasculature or trapping organoids and spheroids in the middle wells in the absence of a gel. Wider channels (A001-2) are recommended for AngioTEER measurements of tissue barriers. Bi directional interstitial flow in the gel matrix is achieved with the IFlowRocker, removing the need for pumps. The open well design allows easy access for pipetting and tissue removal, facilitating down stream analysis. The optically clear plate bottom supports high content imaging with confocal microscopy and highthroughput analysis with plate readers. The 384 well design minimizes the use of cells and reagents, significantly reducing experimental costs per data point. To learn more, visit www.organo-biotech.com/posters.
| Product Code | A002; A002-2 |
|---|---|
| Number of Cultures per Plate | 128 |
| Sterilization Method | UV sterilized |
| Storage Condition | Room temperature (15-25 °C) |
| Storage Time | 6 Months |
| Plate Format | SBS standard 384 well plate |
| Materials | Top plate: optical quality, low compound-absorbing plastic |
| Perfusion | Gravity-driven perfusion with IFlowRocker (B001) |
| Applications | Perfused 3D tissue culture |
| Readouts | – Sensor (TEER with AngioTEER, B002, suitable only with A001-2); |
| Cat Number (#) | A001, A001-2 |
