IFlowPlate™384

$890.00 CAD

IFlowPlate is an organ on a chip platform designed to replicate the complex microenvironment of human tissues with interstitial flow. Each IFlowPlate consists of 128 independent tissue culture units, offering the highest throughput available in the market and compatibility with automation workflows. Each unit includes an inlet, middle, and outlet well, interconnected by two channels. The simplistic design makes the platform highly versatile. To create an epithelial tissue barrier model, a hydrogel can be cast in the middle well, with epithelial cells seeded on the gel surface and supporting cells embedded within. For controlling epithelial topography, TopoStamp (B003) introduces specific patterns on the gel surface. For vascularized organoids, endothelial cells and organoids can be embedded in the gel.

SKU: A001; A001-2 Category:

For simple perfusion cultures of free floating organoids or tissue spheroids, the organoids can be directly seeded into the middle wells without a gel. The dimensions of the inlet and outlet channels are adjustable for specific applications. Narrower channels (A001) are ideal for physically connecting to self assembled vasculature or trapping organoids and spheroids in the middle wells in the absence of a gel. Wider channels (A001-2) are recommended for AngioTEER measurements of tissue barriers. Bi directional interstitial flow in the gel matrix is achieved with the IFlowRocker, removing the need for pumps. The open well design allows easy access for pipetting and tissue removal, facilitating down  stream analysis. The optically clear plate bottom supports high content imaging with confocal microscopy and highthroughput analysis with plate readers. The 384 well design minimizes the use of cells and reagents, significantly reducing experimental costs per data point. To learn more, visit www.organo-biotech.com/posters.

  • For non-commercial research use only.
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  • All prices mentioned are in Canadian Dollar (CAD)
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  • This product is also available on Cedarlane

Specifications

Product Code

A002; A002-2

Number of Cultures per Plate

128

Sterilization Method

UV sterilized

Storage Condition

Room temperature (15-25 °C)

Storage Time

6 Months

Plate Format

SBS standard 384 well plate

Materials

Top plate: optical quality, low compound-absorbing plastic

Perfusion

Gravity-driven perfusion with IFlowRocker (B001)

Applications

Perfused 3D tissue culture

Readouts

– Sensor (TEER with AngioTEER, B002, suitable only with A001-2);
– Imaging (phase contrast, widefield fluorescence, confocal);
– Plate reader (absorption, fluorescence, luminescence);
– Off plate (Histology, ELISA, RNA/DNA analysis, MS, biochemistry)

Key Features

128 tissue culture units

Pump-free bidirectional perfusion

Membrane-free 3D tissue culture in hydrogel matrix

Customizable inlet/outlet channel dimensions

Automation compatible

Low cell and media consumption

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